Protocol

 Copyright 2000-2009 ⓒ NucleoGen Inc. All Rights Reserved.
Contact us
nucleogen@hanmail.net for more information
Tel:031-315-6644     Fax:031-312-2008

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Spin and Vacuum column type and 96 well plate type

Pure DNA, free from contaminants and enzyme inhibitors

Rapid purification of ready-to-use DNA

No organic extraction, no ethanol precipitation

Excellent reproducibility

DNA recovery is 85% - 95% on average

A260/280 ratio is > 1.8

Animal tissues and cells, yeast, bacteria, viral DNA, body fluids, lymphocytes, cultured cells, tissue or buccal swabs

Contained Proteinase K (RNase A Solution : optional)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The Nucleogen Genomic Cell/Tissue Spin Kit provides rapid isolation of genomic DNA from a wide range of organisms and fresh and frozen animal tissues.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Mini

50 preps … 1545

200 preps … 1542

Midi

20 prep …

50 prep …

100 prep …

4 x 96 Plates ………

25 x 96 Plates ………

50 x 96 Plates … 2 x

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Principle

The Nucleogen Genomic Cell/Tissue Spin Kit uses advanced silica gel membrane technology for rapid and efficient isolation of total cellular DNA without phenol, chloroform, or ethanol precipitation. Mechanical homogenization is not necessary since tissues are directly lysed by Proteinase K, reducing hands-on time. The buffer system is optimized to allow selective binding of DNA to the Nucleogen Genomic Cell/Tissue Spi membrane. Simple centrifugation processing completely removes contaminants and enzyme inhibitors such as proteins, divalent cations, and secondary metabolites.


Procedure

For convenience and reduced hands-on time, cells are directly lysed with Proteinase K (supplied) without the need for mechanical homogenization. Lysates are loaded onto Nucleogen Genomic Cell/Tissue spin columns. During a brief spin the DNA selectively binds to the silica gel membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use. After lysis, DNA is purified in as little as 20 minutes in a convenient spin column format. Special protocols and guidelines are provided for different samples, such as paraffin-embedded tissue, blood, cultured cells, rodent tails, insects, bacteria, and yeast.


Applications

Nucleogen Genomic Cell/Tissue Spin Kit purified DNA is free from contaminants and enzyme inhibitors.

 

 

 

 

 

 

 

 

 

 

 

 

Amplification (PCR)
Blotting & Hybridization
Restriction digest
Viral diagnostics
Bacterial diagnostics
Fungal diagnostics
HLA typing
Cancer research
Human genetic testing
Paternity testing
Forensic analysis

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Kit Contents

 Mini

Mini-columns, Collection Tubes, Buffer NL, Buffer NCL, Wash C Solution, Wash D Solution, Elution Buffer, Proteinase K

 Midi

Midi-columns, Collection Tubes, Buffer NL, Buffer NCL, Wash C Solution, Wash D Solution, Elution Buffer, Proteinase K

96 Plate

96 Binding Plate, 96 Elution Plate, Buffer NL, Buffer NCL, Wash C Solution, Wash D Solution, Elution Buffer, Proteinase K, Tape