Protocol

 Copyright 2000-2009 ⓒ NucleoGen Inc. All Rights Reserved.
Contact us
nucleogen@hanmail.net for more information
Tel:031-315-6644     Fax:031-312-2008

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Spin and Vacuum column type and 96 well plate type

Consistent, high yields

Rapid purification of high-quality, ready-to-use DNA

No organic extraction or alcohol precipitation

No phenol/chloroform extraction

Excellent reproducibility

DNA recovery is 85% - 95% on average

A260/280 ratio is > 1.8

Complete removal of contaminants and inhibitors for reliable downstream applications

Fresh and frozen whole blood (with common anticoagulants such as citrate, EDTA, and heparin); plasma; serum; buffy coat; bone marrow; lymphocytes; platelets; body fluids

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Nucleogen Genomic DNA Spin Kit is designed for rapid DNA purification from blood. Using silica gel membrane technology, Nucleogen Genomic DNA Spin Kit deliver high-purity DNA suitable for use in all applications.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Mini

50 preps … 1515

200 preps … 1512

Midi

20 preps … 6520

50 preps … 6550

100 preps … 6500

Maxi

6 preps … 7506

24 preps … 7524

50 preps … 7550

4 x 96 Plates ………

25 x 96 Plates ………

50 x 96 Plates … 2 x

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Principle

The Nucleogen Genomic DNA Spin Kit simplifies isolation of DNA from blood and related body fluids with fast spin column or vacuum procedures. No phenol–chloroform extraction is required. DNA binds specifically to the Nucleogen Genomic DNA Spin silica gel membrane while contaminants pass through. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit. Nucleogen Genomic DNA Spin technology yields genomic viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.

Nucleogen Genomic DNA Spin Kits are supplied with a comprehensive handbook containing protocols for DNA isolation from a number of sample sources.


Procedure

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the Nucleogen Genomic DNA Spin membrane. Alcohol is added and lysates loaded onto the Nucleogen Genomic DNA spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer. The entire process requires only 20 minutes of handling time (lysis times differ according to the sample source).


Applications

Nucleogen Genomic DNA Spin Kits yield DNA sized from 200 bp to 50 kb depending on the age and storage of samples.

 

 

 

 

 

 

 

 

Amplification (PCR)
Blotting & Hybridization
Restriction digest
Viral diagnostics
Bacterial diagnostics
Fungal diagnostics
HLA typing
Cancer research
Human genetic testing
Paternity testing
Forensic analysis

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Kit Contents

 Mini

Mini-columns, Collection Tubes, Buffer NL, Wash C Solution, Wash D Solution, Elution Buffer, Proteinase K

 Midi

Midi-columns, Collection Tubes, Buffer NL, Wash C Solution, Wash D Solution, Elution Buffer, Proteinase K

Maxi

Maxi-columns, Collection Tubes, Buffer NL, Wash C Solution, Wash D Solution, Elution Buffer, Proteinase K

96 Plate

96 Binding Plate, 96 Elution Plate, Buffer NL, Wash C Solution, Wash D Solution, Elution Buffer, Proteinase K, Tape