Protocol

 Copyright 2000-2009 ⓒ NucleoGen Inc. All Rights Reserved.
Contact us
nucleogen@hanmail.net for more information
Tel:031-315-6644     Fax:031-312-2008

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

96 well plate type

100 bp-10 kb DNA size range

Clean-up of DNA up to 10 kb in three easy steps

Up to 90% recovery of ready-to-use DNA

Fast procedure ; Bind-Wash-Elute

> 99.5% primer removal

No removal of mineral oil necessary

Excellent reproducibility

Total procedure is 20-25 min per 96 preparations

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Nucleogen 96 PCR Purification Kit is designed for fast cleanup of DNA fragments from PCR or other enzymatic reactions. Using multiwell technology, Nucleogen 96 PCR Purification Kit deliver high-purity DNA suitable for use in all applications.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

4 x 96 plates ……… 4304

25 x 96 plates ……… 4325

50 x 96 plates … 2 x 4325

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Principle

Nucleogen 96 PCR Purification Kits contain a silica gel membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples. Silica membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.


Procedure

The Nucleogen 96 PCR Purification system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the PCR sample or other enzymatic reaction, and the mixture is applied to the 96-well plate. Nucleic acids adsorb to the silica gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.


Applications

DNA fragments purified with the Nucleogen PCR Purification Kits are ready for direct use in most applications, including:

Automated fluorescent sequencing, including capillary sequencing
Radioactive sequencing
Microarray analysis
Ligation and transformation
Restriction digestion
Labeling
Microinjection
PCR
In vitro transcription

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Kit Contents

96 PCR Binding Plate, 96 Elution Plate, PCR Purification Buffer, Wash A' solution, Elution Buffer, Tape