Protocol

 Copyright 2000-2009 ⓒ NucleoGen Inc. All Rights Reserved.
Contact us
nucleogen@hanmail.net for more information
Tel:031-315-6644     Fax:031-312-2008

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Spin column type

Fast procedure and easy handling

High, reproducible recoveries

Economical Kits

No ethanol or isopropanol precipitation

Total procedure is 5 min

Enzyme removal is > 99.8%

Salt removal is 100%

Dye removal is 100%

Up to 80% recovery of DNA fragments (70 bp - 10 kb)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Nucleogen DNA Clean-up Kit is designed for fast cleanup of DNA fragments from DNA solution. Using silica gel membrane technology, Nucleogen PCR Purification Kit deliver high-purity DNA suitable for use in all applications.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

50 preps … 1415

200 preps … 1412

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Principle

Nucleogen DNA Clean-up Kits contain a silica gel membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples. Silica membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.


Procedure

The Nucleogen DNA Clean-up system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the enzymatic reaction, and the mixture is applied to the Nucleogen DNA Clean-up spin column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.


Applications

DNA fragments purified with the Nucleogen DNA Clean-up Kits are ready for direct use in most applications, including:

Automated fluorescent sequencing, including capillary sequencing
Radioactive sequencing
Microarray analysis
Ligation and transformation
Restriction digestion
Labeling
Microinjection
PCR
In vitro transcription

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                    M    1    2

 

 

 

 

 

 

 

 

Analysis of PCR products before (1) and after (2) purification with the Nucleogen DNA Clean-up Kit (1412).
Sample were analyzed on a 1.2% TAE agarose gel.
M:markers
 

 

 

Kit Contents

Spin Columns, Collection Tubes, DNA Clean-up Buffer, Wash C solution, Elution Buffer