Protocol

 Copyright 2000-2009 ⓒ NucleoGen Inc. All Rights Reserved.
Contact us
nucleogen@hanmail.net for more information
Tel:031-315-6644     Fax:031-312-2008

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Up to 80% recovery of DNA (70 bp - 10 kb)

Gel extraction from standard or low-melt agarose gels in TAE or TBE buffer (contained TBE modifier)

Economical Kits

Excellent reproducibility

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Nucleogen Bead Type (NaI) Gel Extraction technology simplifies the process of purifying DNA into three easy steps: Bind, Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications. The Nucleogen Bead Type (NaI) Gel Extraction Kit is used to purify fragments of DNA from 70 bp to 10 kb.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

200 preps …… 1232

400 preps …… 1234

600 preps …… 1236

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Principle

Nucleogen Bead Type (NaI) Gel Extraction Kits contain a silica bead assembly for binding of DNA in high-chaotropic salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples.


Procedure

The Nucleogen Bead Type (NaI) Gel Extraction system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a high-chaotropic salt buffer and the Nucleogen Bead. Nucleic acids adsorb to the silica bead in the high-chaotropic salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.


Applications

DNA fragments purified with the Nucleogen Bead Type (NaI) Gel Extraction Kits are ready for direct use in most applications, including:

Automated fluorescent sequencing, including capillary sequencing
Radioactive sequencing
Ligation and transformation
Restriction digestion
Labeling
Microinjection
PCR
In vitro transcription

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                 

     M         1          2         3

 

 

 

 

 

 

 

 


2.0 kb -
1.2 kb -
0.8 kb -


0.4 kb -


0.2 kb -

0.1 kb -

 

DNA fragments (size indicated) before extraction with Nucleogen Bead Type (NaI) Gel Extraction Kit (1232) and after extraction.
Lane M: markers ; lane 1 – 3 : after extraction.
Sample were analyzed on a 1.5% Agarose (agarose-1000, Invitrogen) gel in TBE buffer

 

 

Kit Contents

Glass Bead, NaI Solution, TBE Modifier, Wash C solution, Elution Buffer